Data from Tertiary Lymphoid Structures Are Associated with Enhanced Macrophage Activation and Immune Checkpoint Expression and Predict Outcome in Cervical Cancer

• DOI: 10.1158/2326-6066.c.7798963
• Publication Date: 2025-05-02T07:22:28Z
• Authors: Laurent Gorvel; Marylou Panouillot; Marie-Sarah Rouvière; Emilien Billon; Stéphane Fattori; Jumaporn Sonongbua; Nicolas Boucherit; Amira Ben Amara; Olivia Quilichini; Samuel Granjeaud; Clara Degos; Jacques A. Nunès; Xavier Carcopino; Eric Lambaudie; Anne-Sophie Chrétien; Renaud Sabatier; Marie-Caroline Di...; Daniel Olive
• Keywords: Immune checkpoint

<div>Abstract<p>Cervical tumors are usually treated using surgery, chemotherapy, and radiotherapy would benefit from immunotherapies. However, the immune microenvironment in cervical cancer remains poorly described. Tertiary lymphoid structures (TLS) were recently described as markers for better immunotherapy response overall prognosis patients with cancer. We evaluated tumor microenvironment, specifically focusing on TLS, combined high-throughput phenotyping, soluble factor concentration dosage spatial interaction analyses. found that TLS presence was associated a more inflammatory of B cells well activated macrophages dendritic (DC). Furthermore, this myeloid cell activation expression checkpoints, such PD-L1 CD40, proximity conventional type 2 DCs to CD8<sup>+</sup> T cells, indicating interactions control. Finally, we presence, greater B-cell density, DC density improved progression-free survival, substantiating potential prognostic marker. Our results provide evidence denotes target expression.</p></div>