FIGURE 3 from Surgical Inflammation Alters Immune Response to Intraoperative Photodynamic Therapy

• DOI: 10.1158/2767-9764.24119256.v1
• Publication Date: 2023-09-11T14:21:45Z
• Authors: Richard W. Davis; Astero Klampatsa; Gwendolyn M. Cramer; Michele M. Kim; Joann M. Miller; Min Yuan; Cassandra Houser; Emma Snyder; Mary Putt; Sergei A. Vinogradov; Steven M. Albelda; Keith A. Cengel; Theresa M. Busch

<p>TI does not significantly impede direct PDT damage. Clonogenic analysis of tumor tissue immediately (<b>A</b>) or 24 hours (<b>B</b>) after PDT. At 24 hours after TI and/or PDT, reductions were observed in the median number of clonogenic cells per gram of tumor tissue compared with untreated controls (9.3-fold change for TI, 5.9-fold change for PDT, and 4.9-fold change for TI/PDT). Significance was only observed in the TI/PDT group versus untreated tumor control (TC, <i>P</i> = 0.0192). <b>C,</b> Immunoblot analysis of PARP cleavage 24 hours after PDT administration revealed no differences in TI-exposed tumors. <i>n</i> = 4 mice per group. <b>D,</b> Histologic samples taken from TI-, PDT-, or TI/PDT-treated tumors. H&E-stained sections are shown at 10X and 20X magnification. One representative image is shown from three repetitions showing similar extent of necrosis between PDT- and TI/PDT-treated tumors. <i>n</i> = 7–11 mice per group.</p>