FIGURE 5 from Exercise-induced β2-adrenergic Receptor Activation Enhances the Antileukemic Activity of Expanded γδ T-Cells via DNAM-1 Upregulation and PVR/Nectin-2 Recognition

• DOI: 10.1158/2767-9764.25810086.v1
• Publication Date: 2024-05-13T14:22:01Z
• Authors: Forrest L. Baker; Kyle A. Smith; Preetesh L. Mylab...; Tiffany M. Zúñiga; Douglass M. Diak; Helena Batatinha; Grace M. Niemiro; Michael D. Seckeler; Charles R. Pedlar; Daniel P. O'Connor; Jamie Colombo; Emmanuel Katsanis; Richard J. Simpson

<p>Systemic β-AR activation through either acute exercise or ISO infusion evoke similar transcriptomic and surface phenotype shifts among <i>ex vivo</i> expanded Vγ9Vδ2+ T-cells. <b>A,</b> The percentage of activating, inhibitory, and chemokine receptors among expanded Vγ9Vδ2+ T-cells before (REST) and during (EX) exercise or ISO infusion. (<i>n</i> = 8). Statistical significance set as *, <i>P</i> < 0.05. GSEA performed using KEGG and GO terms showing enriched pathways on expanded Vγ9Vδ2+ T-cells that were expanded after (<b>B</b>) exercise or (<b>C</b>) ISO infusion. Graphs show enriched upregulated (blue) and downregulated (red) pathways as well as the percentage of DEGs contributing to each mechanism. Statistical significance set as FDR ≤ 0.25. Data are represented as mean.</p>