FIGURE 3 from Exercise-induced β2-adrenergic Receptor Activation Enhances the Antileukemic Activity of Expanded γδ T-Cells via DNAM-1 Upregulation and PVR/Nectin-2 Recognition

• DOI: 10.1158/2767-9764.25810092.v1
• Publication Date: 2024-05-13T14:22:21Z
• Authors: Forrest L. Baker; Kyle A. Smith; Preetesh L. Mylab...; Tiffany M. Zúñiga; Douglass M. Diak; Helena Batatinha; Grace M. Niemiro; Michael D. Seckeler; Charles R. Pedlar; Daniel P. O'Connor; Jamie Colombo; Emmanuel Katsanis; Richard J. Simpson

<p>Blockade of β₂-AR blunts the mobilization and expansion of Vγ9Vδ2+ T-cells. <b>A,</b> The physiologic effects of a placebo, β1-AR antagonist (10 mg bisoprolol), or a nonselective β1+β2 antagonist (80 mg, nadolol) on VO₂ (mL/minute), systolic blood pressure (mmHg), HR (bpm), epinephrine (ng/mL), and norepinephrine (ng/mL) during the last minutes of acute exercise (<i>n</i> = 9). The effects of a placebo, β1-AR antagonist, or a nonselective β1+β2 antagonist on the total number of Vγ9Vδ2+ T-cells mobilized with exercise (<b>B</b>) and the <i>ex vivo</i> expansion of Vγ9Vδ2+ T-cells after 14 days with ZOL+IL2 (<i>n</i> = 9; <b>C</b>). Data are represented as mean ± SEM; *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001; ****, <i>P</i> < 0.0001 by repeated measures one-way ANOVA with Bonferroni <i>post hoc</i> test.</p>