FIGURE 1 from Exercise-induced β2-adrenergic Receptor Activation Enhances the Antileukemic Activity of Expanded γδ T-Cells via DNAM-1 Upregulation and PVR/Nectin-2 Recognition

• DOI: 10.1158/2767-9764.25810098.v1
• Publication Date: 2024-05-13T14:22:53Z
• Authors: Forrest L. Baker; Kyle A. Smith; Preetesh L. Mylab...; Tiffany M. Zúñiga; Douglass M. Diak; Helena Batatinha; Grace M. Niemiro; Michael D. Seckeler; Charles R. Pedlar; Daniel P. O'Connor; Jamie Colombo; Emmanuel Katsanis; Richard J. Simpson

<p>Exercise preferentially mobilizes γδ T-cells with phenotypic and transcriptomic profiles associated with anti-tumor immunity. <b>A,</b> The percentage or MFI of activating, inhibitory, and chemokine receptors among Vδ2+ and Vδ1+ T-cells before (REST) and during (EX) acute exercise (<i>n</i> = 8). Significance is indicated by * (<i>P</i> < 0.05). <b>B,</b> GSEA performed using KEGG and GO terms showing enriched pathways on exercise mobilized γδ T-cells (<i>n</i> = 3). Graphs show enriched upregulated (blue) and downregulated (red) pathways as well as the percentage of DEGs contributing to each mechanism. Statistical significance set as FDR ≤ 0.25. <b>C,</b> Azimuth map for human PBMCs showing the 26 clusters identified by scRNA-seq at rest; γδ T-cells mapping is circled in red. (<i>n</i> = 3). <b>D,</b> Chord diagram displaying the leading-edge genes driving the enrichment of terms associated with cytotoxicity and anti-tumor signaling in γδ T-cells (<i>n</i> = 3). Data are represented as mean; *, <i>P</i> < 0.05, Students two-tailed paired <i>t</i> test (A); * ≤0.25, FDR (B).</p>