FIGURE 1 from Exercise-induced β2-adrenergic Receptor Activation Enhances the Antileukemic Activity of Expanded γδ T-Cells via DNAM-1 Upregulation and PVR/Nectin-2 Recognition

• DOI: 10.1158/2767-9764.25810098
• Publication Date: 2024-05-13T14:22:56Z
• Authors: Forrest L. Baker; Kyle A. Smith; Preetesh L. Mylab...; Tiffany M. Zúñiga; Douglass M. Diak; Helena Batatinha; Grace M. Niemiro; Michael D. Seckeler; Charles R. Pedlar; Daniel P. O'Connor; Jamie Colombo; Emmanuel Katsanis; Richard J. Simpson

<p>Exercise preferentially mobilizes γδ T-cells with phenotypic and transcriptomic profiles associated anti-tumor immunity. <b>A,</b> The percentage or MFI of activating, inhibitory, chemokine receptors among Vδ2+ Vδ1+ before (REST) during (EX) acute exercise (<i>n</i> = 8). Significance is indicated by * (<i>P</i> < 0.05). <b>B,</b> GSEA performed using KEGG GO terms showing enriched pathways on mobilized 3). Graphs show upregulated (blue) downregulated (red) as well the DEGs contributing to each mechanism. Statistical significance set FDR ≤ 0.25. <b>C,</b> Azimuth map for human PBMCs 26 clusters identified scRNA-seq at rest; mapping circled in red. <b>D,</b> Chord diagram displaying leading-edge genes driving enrichment cytotoxicity signaling Data are represented mean; *, <i>P</i> 0.05, Students two-tailed paired <i>t</i> test (A); ≤0.25, (B).</p>