The Tat Protein of the Caprine Arthritis Encephalitis Virus Interacts with the Notch2 EGF-Like Repeats and the Epithelin/Granulin Precursor

• DOI: 10.1159/000072434
• Publication Date: 2003-08-22T08:19:50Z
• Authors: Nitza Shoham; Limor Cohen; Arnona Gazit; Abraham Yaniv
• Keywords: Repetitive Sequences, Amino Acid; 0303 health sciences; Arthritis-Encephalitis Virus, Caprine; Binding Sites; Base Sequence; Goats; Receptors, Cell Surface; In Vitro Techniques; Cell Line; Protein Structure, Tertiary; 3. Good health; 03 medical and health sciences; Progranulins; Two-Hybrid System Techniques; DNA, Viral; Gene Products, tat; Animals; Humans; Intercellular Signaling Peptides and Proteins; Receptor, Notch2

Using the yeast two-hybrid system, we screened a human placenta cDNA library and identified two proteins that interacted with Tat protein of caprine arthritis encephalitis virus (CAEV): EGF-like repeats 1–6 extracellular domain Notch2 receptor epithelin/granulin growth factor precursor. This interaction was also confirmed in mammalian cells. vitro mutagenesis assays, showed each one three cysteine residues located within cysteine-rich CAEV is essential for binding to both protein. It thus suggested plays role between either or domains, which exhibit EGF-like-repeat-imposed spatial conformation. assumed such interactions might modulate physiological functions epithelin/granulin, thereby affecting various pathologies associated CAEV.