Relationship between Calcium-Activated Chloride Channel 1 and MUC5AC in Goblet Cell Hyperplasia Induced by Interleukin-13 in Human Bronchial Epithelial Cells

• DOI: 10.1159/000091391
• Publication Date: 2006-02-07T09:37:58Z
• Authors: Masanori Yasuo; Keisaku Fujimoto; Tsuyoshi Tanabe; Hironobu Yaegashi; Kenji Tsushima; Keiichirou Takasuna; Takeshi Koike; Mutsuo Yamaya; Toshio Nikaido
• Keywords: Niflumic acid; Goblet cell; Chloride channel

<i>Background:</i>Interleukin (IL)-13 has recently been reported as the major T-helper 2 cytokine involved in mucus overproduction and oversecretion allergic airways. However, relationship between human calcium-activated chloride channel-1 (hCLCA1) MUC5AC induced by IL-13 vitro not fully investigated. <i>Objectives:</i> The present study examines whether induces expression of hCLCA1 normal bronchial epithelial (NHBE) cells. We also investigated development goblet cell hyperplasia (GCH). <i>Methods:</i> NHBE cells were isolated from bronchi, cultured with an air-liquid interface. gene protein expression, well GCH examined after exposure to IL-13. <i>Results:</i> Incubation for 14 21 days increased total number cells, periodic acid-Schiff (PAS)-stained mRNA MUC5AC. secretory granules incubation Niflumic acid, a channel inhibitor, reduced MUC5AC, PAS-positive exposed or expressed receptor α<sub>1</sub> (IL-13Rα<sub>1</sub>), antibody Rα<sub>1</sub> <i>Conclusions:</i> might induce well-differentiated These differentiate into become hyperplastic.