Reactivity of Synthetic SAG1 (p30) Peptide Sequences with RH, S273 and Beverley Strain-Induced Anti-<i>Toxoplasma gondii</i> Antibodies

• DOI: 10.1159/000101051
• Publication Date: 2007-05-14T08:50:48Z
• Authors: M. Kato; F.G. Claveria; Y. Maki; K. Sanda; T. Tanaka; Y. Omata; H. Nagasawa; N. Suzuki
• Keywords: Strain (injury)

We compared the reactivity of IgG1 and IgG2a antibodies in mouse sera after infection with virulent RH low-virulent S273 Beverley strains Toxoplasma gondii against SAG1 recombinant p30 (rp30) synthetic peptides.Infected serum samples were collected 9 days infection, level total IgG, rp30 protein twenty peptides assessed. The glycosylphosphatidylinositol (GPI) modification site, hydrophilic-hydrophobic structure, transmembrane region secondary structure sequence analyzed using software.The strain-infected mice produced a higher but lower antigen, while than strain. difference between strain was largely confined to amino acid positions 291-336, showing mutations GPI anchor site.The IgG antigen points importance primary assumed by antigens activation Th cells and, subsequently, induction its subclasses.