Nebivolol Induces a Hyperpolarizing Effect on Smooth Muscle Cells in the Mouse Renal Artery by Activation of Beta-2-Adrenoceptors

• DOI: 10.1159/000110011
• Publication Date: 2007-10-22T13:07:57Z
• Authors: A. Georgescu; F. Pluteanu; M.-L. Flonta; E. Badila; M. Dorobantu; D. Popov
• Keywords: Myograph; Nebivolol; Iberiotoxin; Electrical impedance myography

Nebivolol is a highly selective beta(1)-adrenoceptor antagonist with vasodilator properties involving the vascular endothelium, but its effect on smooth muscle cells (SMC) still unclear. In this paper, we tested of nebivolol renal artery and investigated cellular mechanism involved. To purpose, denuded arteries isolated from mice were studied in vitro using myograph nitric oxide (NO) sensor techniques, while SMC culture analyzed by patch-clamp technique. The technique was used to assay arterial muscular layer, establish optimal dose drug be single Using both examined potential contribution beta(2)-adrenoceptors Ca(2+)-activated K(+) channels nebivolol-induced effects, exposing cultures specific inhibitors such as butoxamine (100 micromol/l), tetraethylammonium (TEA, 1 mmol/l), iberiotoxin nmol/l). direct measurement NO enabled us evaluate if induces/or not release arteries. results study show that exerts effects maximal response achieved at concentration 50 micromol/l. involve binding subsequent activation SMC, no NO. Taken together, brings new insights into underlying vasodilation.