Oncogenic Transformation by Cellular DNA Isolated from Human Cytomegalovirus-lnfected Cells

• DOI: 10.1159/000150264
• Publication Date: 2017-04-18T21:25:24Z
• Authors: I. Boldogh; E.-S. Huang; J.F. Baskar; L. Gergely; T. Albrecht
• Keywords: 0301 basic medicine; Fibrosarcoma; Cytomegalovirus; Transfection; Virus Replication; Cell Line; 3. Good health; Molecular Weight; 03 medical and health sciences; Cell Transformation, Neoplastic; Phenotype; Cricetinae; DNA, Viral; Animals; Humans

Delayed replication of human cytomegalovirus (CMV) was initiated in embryonic fibroblasts using partially ultraviolet light-inactivated virus stock. Cellular [high molecular weight (HMW)] DNA extracted from CMV-infected cell cultures demonstrated a substantial increase transforming activity after introduction into hamster embryo relative to HMW mock-infected cells. The varied between 0.01 and 0.25 foci/micrograms DNA. isolated cells initiation viral synthesis significant decrease the induction morphologically transformed foci. unaffected by HindIII or XbaI endonuclease digestion, but it sensitive sonication EcoRI DNase treatment. Six lines were established foci altered Cells these loss contact inhibition, semisolid agarose medium containing low serum, high saturation density, tumorigenicity when implanted hamsters. Histopathological examination tumors identified tissues as fibrosarcomas. In situ hybridization Southern blot analysis either did not demonstrate presence sequences with homology region entire probe. lack well similar phenotypic characteristics suggest that alteration(s) induced CMV may occur specific region(s) cellular