Determination of Circulating Endothelial Cells and Endothelial Progenitor Cells Using Multicolor Flow Cytometry in Patients with Thrombophilia

• DOI: 10.1159/000499524
• Publication Date: 2019-04-17T21:00:57Z
• Authors: Martin Řádek; Eva Babuňková; Martin Špaček; Tomáš Kvasnička; Jan Kvasnička
• Keywords: CD146; Endothelial progenitor cell; Endothelial Dysfunction

Endothelial progenitor cells (EPCs) and circulating endothelial (CECs) have been described as markers of damage dysfunction in several diseases, including deep venous thrombosis. Their role patients with known thrombophilia has not yet evaluated. Both EPCs CECs represent extremely rare cell populations. Therefore, it is essential to use standardized methods for their identification quantification.In this study, we used multicolor flow cytometry analyze the number or without a history Patients hematological malignancies after high-dose chemotherapy acute myocardial infarction were positive controls.EPC CEC immunophenotypes determined CD45dim/-CD34+CD146+CD133+ CD45dim/-CD34+CD146+CD133-, respectively. Increased levels observed control groups. No significant changes detected compared healthy controls.Our optimized method allows unambiguous quantification peripheral blood. Our results support previous studies showing that elevated could serve an indicator injury dysfunction. Normal found thrombophilia.