Isolation and Screening of Potential Cellulolytic and Xylanolytic Bacteria from Soil Sample for Degradation of Lignocellulosic Biomass

• DOI: 10.11594/jtls.06.03.06
• Publication Date: 2017-01-30T04:36:05Z
• Authors: Bhupal Govinda Sh...; Sanjaya Ghimire; Shakep Bhattarai; Sailesh Phuyal; Bimal Thapa
• Keywords: Lignocellulosic Biomass; Bagasse

Cellulolytic/Xylanolytic microorganisms such as bacteria and fungi are accountable for conversion of lignocellulosic biomass in soil. Despite this vast number cellulose/xylanase producers, there is a deficiency that can produce significant amount cellulase/xylanase enzyme to proficiently degrade cellulose/xylan fermentable sugars. Although have extremely high natural diversity, which bestowsthem with the aptitude stable enzymes, little emphasis has been given production from bacteria. Seven soil samples were collected eastern hilly districts Nepal viz. Taplejung, Panchthar Sankhuwasabha districts, surface at depth 10cm 20cm, isolated separately. From seven samples, four bacterial isolates obtained. Isolates (PSS, P1D, TLC, SNK) then screened cellulolytic/xylanolytic activity using Congo red assay on Carboxymethylcellulose (CMC)/xylan agar plates. The obtained was dependent substrate concentration. enzymes produced by also measured compared pretreated sugarcane bagasse. Among those greatest zone inhibition both CMC (1.3 cm) xylan (1.0 media seen isolate P1D. It highest endoglucanase xylanase i.e. 0.035 U/mL 0.050 respectively 0.010 mg mL-1 standard concentration xylan.Â