Real-time measurement of non-lethal platelet thromboembolic responses in the anaesthetized mouse

• DOI: 10.1160/th07-07-0479
• Publication Date: 2008-01-22T08:36:57Z
• Authors: Charalambos Tymvios; Sarah Jones; Christopher Moore; Simon C. Pitchford; Clive P. Page; Michael Emerson
• Keywords: Bleeding time

Summary Identifying and evaluating new therapeutic targets in platelets requires advanced animal models which platelet responses can be measured directly situ.This is important because function strongly influenced by external factors such as those originating from the vascular endothelium. Our objectives were to record graded, non-lethal thromboembolic agonists situ mouse demonstrate an inhibitory effect of aspirin our model. Radiolabelled infused into anaesthetized mice ADP, collagen thrombin changes associated counts miniaturized detection probes placed over thoracic region. All induced dose-dependent due accumulation thrombi pulmonary vasculature. We confirmed a specific comparing erythrocyte showing aggregates lung vasculature histologically. Simultaneous injection adrenaline increased protracted synergistic compared with individual these agents inhibited collagen-induced responses. clinical relevance model that thromboembolism mouse, like embolism humans, impaired cardiovascular performance. present refined method for measuring agonist dose-responses real-time without inducing mortality mouse. technique will useful investigating molecular determinants physiological pathophysiological in-vivo context enable investigations both non-platelets mediators thrombus formation.