Excessive Tumor Necrosis Factor Activation After Infarction Contributes to Susceptibility of Myocardial Rupture and Left Ventricular Dysfunction

• DOI: 10.1161/01.cir.0000147233.10318.23
• Publication Date: 2004-11-09T01:14:07Z
• Authors: Mei Sun; Fayez Dawood; Wen-Hu Wen; Manyin Chen; Ian Dixon; Lorrie A. Kirshen...; Peter P. Liu
• Keywords: Mice, Knockout; Tumor Necrosis Factor-alpha; Myocardium; Heart Rupture; Myocardial Infarction; NF-kappa B; Transcription Factor RelA; Apoptosis; Extracellular Matrix; 3. Good health; Mice, Inbred C57BL; Mice; Myocarditis; Random Allocation; 03 medical and health sciences; 0302 clinical medicine; Gene Expression Regulation; Matrix Metalloproteinase 9; Enzyme Induction; Animals; Cytokines; Matrix Metalloproteinase 2; Collagen

Background—

We investigated the potential contributions of tumor necrosis factor-α (TNF-α) on the incidence of acute myocardial rupture and subsequent chronic cardiac dysfunction after myocardial infarction (MI) in TNF knockout (TNF −/− ) mice compared with C57/BL wild-type (WT) mice.

Methods and Results—

Animals were randomized to left anterior descending ligation or sham operation and killed on days 3, 7, 14, and 28. We monitored cardiac rupture rate, cardiac function, inflammatory response, collagen degradation, and net collagen formation. We found the following: (1) within 1 week after MI, 53.3% (n=120) of WT mice died of cardiac rupture, in contrast to 2.5% (n=80) of TNF −/− mice; (2) inflammatory cell infiltration and cytokine expression were significantly higher in the infarct zone in WT than TNF −/− mice on day 3; (3) matrix metalloproteinase-9 and -2 activity in the infarcted myocardium was significantly higher in WT than in TNF −/− mice on day 3; (4) on day 28 after MI compared with sham, there was a significant decrease in LV developed pressure (74%) and ±dP/dt max (68.3%/65.3%) in WT mice but a less significant decrease in ±dP/dt max (25.8%/28.8%) in TNF −/− mice; (5) cardiac collagen volume fraction was lower in WT than in TNF −/− mice on days 3 and 7 but higher on day 28 compared with TNF −/− mice; and (6) a reduction in myocyte apoptosis in TNF −/− mice occurred on day 28 compared with WT mice.

Conclusions—

Elevated local TNF-α in the infarcted myocardium contributes to acute myocardial rupture and chronic left ventricle dysfunction by inducing exuberant local inflammatory response, matrix and collagen degradation, increased matrix metalloproteinase activity, and apoptosis.