Activation of Hypoxia-Inducible Factor-1 in Bacillary Angiomatosis

• DOI: 10.1161/01.cir.0000155608.07691.b7
• Publication Date: 2005-02-22T01:14:30Z
• Authors: Volkhard A.J. Kempf; Maria Lebiedzieje...; Kari Alitalo; Joo-Hee Wälzlein; Urs Ehehalt; Jeannette Fiebig; Stephan Huber; Burkhardt Schütt; Christian A. Sander; Steffen Müller; Guntram Grassl; Amir S. Yazdi; Bernhard Brehm; Ingo B. Autenrieth
• Keywords: 0301 basic medicine; Umbilical Veins; Bartonella henselae; Neovascularization, Pathologic; Macrophages; Endothelial Cells; Nuclear Proteins; Histiocytes; Hypoxia-Inducible Factor 1, alpha Subunit; Immunohistochemistry; Cell Hypoxia; Up-Regulation; 3. Good health; DNA-Binding Proteins; 03 medical and health sciences; Adenosine Triphosphate; Fimbriae, Bacterial; Angiomatosis, Bacillary; Humans; Endothelium, Vascular; Hypoxia-Inducible Factor 1; HeLa Cells; Transcription Factors

Background—

Bartonella species are the only known bacterial pathogens causing vasculoproliferative disorders in humans (bacillary angiomatosis [BA]). Cellular and bacterial pathogenetic mechanisms underlying the induction of BA are largely unknown.

Methods and Results—

Activation of hypoxia-inducible factor-1 (HIF-1), the key transcription factor involved in angiogenesis, was detected in Bartonella henselae –infected host cells in vitro by immunofluorescence, Western blotting, electrophoretic mobility shift, and reporter gene assays and by immunohistochemistry in BA tissue lesions in vivo. Gene microarray analysis revealed that a B henselae infection resulted in the activation of genes typical for the cellular response to hypoxia. HIF-1 was essential for B henselae –induced expression of vascular endothelial growth factor as shown by inhibition with the use of HIF-1–specific short-interfering RNA. Moreover, infection with B henselae resulted in increased oxygen consumption, cellular hypoxia, and decreased ATP levels in host cells. Infection with a pilus-negative variant of B henselae did not lead to cellular hypoxia or activation of HIF-1 or vascular endothelial growth factor secretion, suggesting a crucial role of this bacterial surface protein in the angiogenic reprogramming of the host cells.

Conclusions—

B henselae induces a proangiogenic host cell response via HIF-1. Our data provide for the first time evidence that HIF-1 may play a role in bacterial infections.