Effect of endothelin-1 on neutrophil adhesion to endothelial cells and perfused heart.

• DOI: 10.1161/01.cir.88.3.1166
• Publication Date: 2012-06-12T00:01:09Z
• Authors: A López Farré; A Riesco; G Espinosa; E Digiuni; M R Cernadas; V Alvarez; M Montón; F Rivas; M J Gallego; J Egido
• Keywords: Male; 0301 basic medicine; Neutrophils; Endothelins; Macrophage-1 Antigen; Heart; In Vitro Techniques; Lymphocyte Function-Associated Antigen-1; 3. Good health; Perfusion; 03 medical and health sciences; Cell Adhesion; Animals; Humans; Cattle; Endothelium, Vascular; Rabbits; Cell Adhesion Molecules; Cells, Cultured; Peroxidase

BACKGROUND Based on recent evidence showing that endothelin-1 stimulates several activation mechanisms neutrophils, the aim of present study was to analyze effects neutrophil adhesion endothelial cells and accumulation in heart. METHODS AND RESULTS The experiments included (1) 51Cr-labeled human neutrophils bovine culture both presence absence monoclonal antibodies against alpha- beta-subunits integrins; (2) surface expression beta-integrin antigens; (3) isolated perfused rabbit heart; (4) vivo autologous heart, as assessed by myeloperoxidase activity. Endothelin-1 stimulated (increase 1 x 10(5) +/- 10(4) per well). endothelin-1-induced blocked (83 6%) anti-CD18 antibody TS1/18 anti-alpha-subunit antibodies. CD18 CD11b also increased endothelin-1. enhanced heart 4.2 times throughout a TS1/18-inhibitable mechanism. Myeloperoxidase activity hearts infused with CONCLUSIONS an effect adhesive molecules surface. vitro Antibodies integrin complex block endothelin-1-dependent adhesion. These findings have potential importance pathophysiology endothelin-1-increased states.