Pathophysiological correlates of increased serum tumor necrosis factor in patients with congestive heart failure. Relation to nitric oxide-dependent vasodilation in the forearm circulation.

• DOI: 10.1161/01.cir.90.1.12
• Publication Date: 2012-06-12T00:34:42Z
• Authors: S D Katz; R Rao; J W Berman; M Schwarz; L Demopoulos; R Bijou; T H LeJemtel
• Keywords: Brachial artery; Plethysmograph; Pathophysiology

BACKGROUND Tumor necrosis factor-alpha (TNF alpha), which we and others have shown to be elevated in patients with severe congestive heart failure (CHF), is involved the regulation of nitric oxide metabolism. Whether increased concentrations TNF alpha affect oxide-mediated vasodilation CHF has not been studied previously. METHODS AND RESULTS Serum alpha, interleukin-1 (IL-1), interleukin-2 (IL-2), interleukin-6 (IL-6) were determined venous blood (pg/mL) from 17 stable New York Heart Association classes II III (mean age, 58 +/- 11 years; mean left ventricular ejection fraction, 19.5 7.3) age-matched normal subjects enzyme-linked immunosorbent assays (detection limit assays, 20 pg/mL). Forearm flows plethysmography (mL/min per 100 mL) 7 response brachial artery administration graded acetylcholine (10(-6) mol/L 10(-5) mol/L) nitroglycerin (10(-7) 10(-6) mol/L). above detection limits assay 10 serum concentration, 39.4 3.8 flow responses significantly greater these than without detectable closely correlated (r > or = .81, P < .01 r .65, .05 respectively). In 1 subjects, concentration was just assay. IL-2 14 112 19 detected subjects. IL-1 IL-6 below all assayed. CONCLUSIONS Increased are forearm regional nitroglycerin. The significant correlation between suggests that both inducible constitutive forms synthase peripheral vasomotor tone CHF.