Myocardial Osteopontin Expression Is Associated With Left Ventricular Hypertrophy

• DOI: 10.1161/01.cir.96.9.3063
• Publication Date: 2012-06-12T00:41:02Z
• Authors: Kristof Graf; Yung S. Do; Naoto Ashizawa; Woerner P. Meehan; Cecilia M. Giachelli; Charles C. Marboe; Eckart Fleck; Willa A. Hsueh
• Keywords: Atrial natriuretic peptide; Myocardial fibrosis; Osteopontin

Osteopontin (OP) has been identified in cultured rat cardiac fibroblasts, where it contributes to angiotensin II (AII)-induced remodeling processes; cardiomyocytes; and macrophages tissues with inflammation. However, the presence of OP not reported histological sections myocardial tissue. In present study, we investigated (1) regulation mRNA expression (2) localization neonatal adult normal hypertrophied hearts; (3) histology specimens from humans either myocyte hypertrophy or no pathological changes.Cultured cardiomyocytes expressed were immunoreactive for OP. Endothelin-1 (ET-1) norepinephrine (NE) increased both atrial natriuretic peptide (ANP) levels twofold threefold (P<.01). was prominent ventricular tissue rats renovascular hypertension aortic banding, whereas barely detectable observed ANP ventricles correlated (r2=.87, P<.001). immunoreactivity transcripts predominantly found associated inflammatory cells hearts. No staining Human myocardium extensive fibrosis cardiomyocyte obtained explanted hearts idiopathic (n=5) ischemic cardiomyopathy (n=7) demonstrated substantial right left that leukocyte infiltration. situ hybridization as major source these contrast, four five endomyocardial biopsies histology.The study provides first evidence are a vivo suggests induction is strongly hypertrophy.