“Apoptotic” Myocytes in Infarct Area in Rabbit Hearts May Be Oncotic Myocytes With DNA Fragmentation

• DOI: 10.1161/01.cir.98.14.1422
• Publication Date: 2012-06-12T00:41:02Z
• Authors: Michiya Ohno; Genzou Takemura; Atsuko Ohno; Jun Misao; Yukihiro Hayakawa; Shinya Minatoguchi; Takako Fujiwara; Hisayoshi Fujiwara
• Keywords: Fragmentation

Background —Modes of cell death have been defined morphologically as apoptosis and oncosis. Infarcted myocytes reported to show apoptosis, revealed by DNA fragmentation ladder in situ terminal deoxynucleotidyl transferase–mediated dUTP nick end-labeling (TUNEL) at the light microscopic level. We investigated whether TUNEL-positive infarcted apoptotic or oncotic ultrastructures using electron TUNEL, which can simultaneously observe ultrastructure same myocytes. Methods Results —Thirty rabbits were divided into 5 groups (n=6 each) that subjected a sham operation 30-minute ischemia followed 0-minute, 30-minute, 2-hour, 4-hour reperfusion coronary artery. In 2- only, electrophoresis showed pattern, TUNEL finding was positive nuclei localized area (6±2% 11±3%, respectively). Electron with significant accumulation immunogold particles (indicating an electronic TUNEL–positive result) observed only irreversibly found hearts (41±3% 83±4%, Irreversibly (indicated swelling, inhomogeneously clumped chromatin nuclei, dense bodies mitochondria, and/or ruptured plasma membranes) also seen 0- groups, did not exhibit There no evidence Conclusions —DNA occurs had already shown oncotic, but apoptotic, reperfusion. Therefore, itself does always mean so-called may belong category other than apoptosis.