Human Endothelium: Target for Aldosterone

• DOI: 10.1161/01.hyp.0000123572.45556.a5
• Publication Date: 2004-03-02T01:56:29Z
• Authors: Hans Oberleithner; Thomas Ludwig; Christoph Riethmu...; Uta Hillebrand; Lars Albermann; Claudia Schäfer; Victor Shahin; Hermann Schillers
• Keywords: Amiloride; Mineralocorticoid; Epithelial sodium channel

Aldosterone has long been known to control water and electrolyte balance by acting on mineralocorticoid receptors in kidney. However, recent studies demonstrated the presence of these nonclassical locations, including cardiovascular system. We tested hypothesis whether endothelial cells respond aldosterone with changes cell volume, a measure for ion-mediated movement across membrane. By means atomic force microscopy fluid, we measured volume adherent human umbilical venous exposed 72 hours 10 nmol/L aldosterone. Over this period time, swell approximately 18%. Aldosterone-induced swelling is prevented 100 receptor antagonist spironolactone, added primary culture. Aldosterone-treated dramatically shrink when 1 micromol/L diuretic amiloride applied. Cells deprived do not amiloride. Our conclusions are: (1) leads sustained inhibited administration spironolactone or sodium channel blocker amiloride; (2) after exposure; (3) renal diuretics act cells; (4) both could be useful medical applications prevent aldosterone-mediated dysfunction.