Abnormalities in growth characteristics of aortic smooth muscle cells in spontaneously hypertensive rats.

• DOI: 10.1161/01.hyp.13.6.589
• Publication Date: 2012-06-12T00:23:24Z
• Authors: V Hadrava; J Tremblay; P Hamet
• Keywords: Thymidine; Platelet-derived growth factor; Spontaneously hypertensive rat

Comparative studies have shown that cultured vascular smooth muscle cells from spontaneously hypertensive rats (SHR) proliferate to a higher cell number, grow greater density, and specific growth rate, particularly at saturation than those of the normotensive Wistar-Kyoto (WKY) control rats. The difference was not due varying survival nor attachment ability after passage. degree DNA synthesis estimated by [3H]thymidine incorporation into newly synthesized DNA. uptake increased with escalating concentrations calf serum reached plateau 5% in WKY rats, whereas an excessive, continuous rise observed SHR up 20% concentration. tested stimulation platelet-derived factor epidermal factor. A significantly amount noted when were stimulated or alone, their simultaneous addition did change 50% effective concentration but heightened maximal response. These data provide evidence aortic proliferation aortas mitogen suggest defect stimulatory-inhibitory control.