Gene expression and tissue localization of the two isoforms of angiotensin I converting enzyme.

• DOI: 10.1161/01.hyp.21.6.827
• Publication Date: 2012-06-12T00:24:03Z
• Authors: M Sibony; J M Gasc; F Soubrier; F Alhenc-Gelas; P Corvol
• Keywords: Convoluted tubule

Angiotensin converting enzyme exists in two different isoforms, somatic and germinal, whose respective distributions intracellular localizations have not been precisely determined. The differing biochemical molecular characteristics of the isozymes allowed preparation antibodies specific for each angiotensin isoforms nucleic acid probes, one which was germinal isoform. Immunohistochemistry situ hybridization were used to determine cell distribution of, respectively, their corresponding messenger RNAs classically studied tissues male adult humans marmosets. Results provided by methods always concordant identical species. form expressed uniquely (vascular endothelial cells at brush border renal proximal convoluted tubule, jejunal villus, epididymal duct epithelia), with a precise stage-specific pattern, starting round spermatids finishing spermatozoa. In documented presence RNA tubule epithelium, enterocytes, epithelium demonstrated that there no direct correlation between levels it encodes for, i.e., enzyme, given epithelium. significance ultraselective expression its very stage spermatogenesis remains uncertain.