Regulation of Endothelial Production of C-Type Natriuretic Peptide in Coculture With Vascular Smooth Muscle Cells

• DOI: 10.1161/01.res.78.4.606
• Publication Date: 2012-06-12T00:47:05Z
• Authors: Yasato Komatsu; Hiroshi Itoh; Shin-ichi Suga; Yoshihiro Ogawa; Norio Hama; Ichiro Kishimoto; Osamu Nakagawa; Toshio Igaki; Kentaro Doi; Takaaki Yoshimasa; Kazuwa Nakao
• Keywords: Atrial natriuretic peptide

Abstract Recently, we have demonstrated that C-type natriuretic peptide (CNP) is produced in vascular endothelial cells (ECs). In the present study, investigated interaction of ECs and smooth muscle (SMCs) for production CNP its action on growth, using EC/SMC coculture system. The concentration CNP-like immunoreactivity medium was increased 60-fold within 48 hours with direct contact compared EC alone. Northern blot analysis revealed augmented expression mRNA coculture. accumulation intracellular cGMP concomitantly increased, this response blocked by anti-CNP monoclonal antibody HS-142-1, a nonpeptide atrial receptor antagonist. biologically active transforming growth factor-β (TGF-β) culture SMCs elevated to level stimulate CNP. Actually, neutralizing against TGF-β abrogated These results show at least part regulated TGF-β. Furthermore, conditioned from stimulated growth-inhibitory effect SMCs, which abolished HS-142-1. treatment HS-142-1 also significantly cell number study reveals pathophysiological significance as paracrine/autocrine regulator SMCs.