Cloning and Characterization of α1H From Human Heart, a Member of the T-Type Ca2+Channel Gene Family

• DOI: 10.1161/01.res.83.1.103
• Publication Date: 2012-06-12T00:47:05Z
• Authors: Leanne L. Cribbs; Jung-Ha Lee; Jie Yang; Jonathan Satin; Yi Zhang; Asif Daud; Jane Barclay; Magali P. Williamson; Margaret Fox; Michele Rees; Edward Perez-Reyes
• Keywords: Mibefradil; Calcium-activated potassium channel; Cloning (programming)

Voltage-activated Ca2+ channels exist as multigene families that share common structural features. Different are distinguished by their electrophysiology and pharmacology can be classified either low or high voltage-activated channels. Six alpha1 subunit genes cloned previously code for channels; therefore, we have used a database search strategy to identify new channel genes, possibly including (T-type) A novel expressed sequence-tagged cDNA clone of alpha1G was screen library, in the present study, report cloning alpha1H (or CavT.2), from human heart. Northern blots mRNA detected more expression peripheral tissues, such kidney heart, than brain. We mapped gene, CACNA1H, chromosome 16p13.3 mouse 17. Expression HEK-293 cells resulted currents displaying voltage dependence, kinetics, unitary conductance characteristic native T-type The is sensitive mibefradil, nondihydropyridine blocker, with an IC50 1.4 micromol/L, consistent reported potency mibefradil Together alpha1G, rat brain also our laboratory, these define unique family