Brain Capillary Tissue Plasminogen Activator in a Diabetes Stroke Model

• DOI: 10.1161/01.str.27.4.712
• Publication Date: 2011-06-17T20:08:03Z
• Authors: Mamoru Kittaka; Liang Wang; Ning Sun; Steven S. Schreiber; Nicholas W. Seeds; Mark Fisher; Berislav V. Zlokovic
• Keywords: Stroke; Cerebral edema

Background and Purpose Tissue plasminogen activator (TPA) is normally expressed in rat brain capillaries. This study examines the expression of TPA capillaries diabetic rats relation to focal ischemic injury. Methods Diabetes type 1 was induced by streptozotocin for 7 days. Acute hyperglycemia 50% dextrose. Expression determined Western blot reverse transcription-polymerase chain reaction analyses. Focal stroke produced hour reversible middle cerebral artery occlusion. Physiological variables blood flow were monitored during occlusion within reperfusion. Neurological neuropathologic examinations performed after 24 hours Results All developed comparable (≈15 mmol/L). A complete depletion protein 6.5-fold decrease mRNA found rats, contrast normal capillary levels hyperglycemic rats. The periphery core significantly reduced reperfusion 52% 62% ( P <.001) 23% 25% <.05) neurological score worsened 3.2-fold <.0003) diabetes 24% only. Significant 41% <.007) 29% increases infarct volume 163% 60% edema relative control respectively. Conclusions 1, but not acute hyperglycemia, produces downregulation reduction associated with impaired restoration an insult, poor outcome, enhanced