Mice Deficient in Mac-1 (CD11b/CD18) Are Less Susceptible to Cerebral Ischemia/Reperfusion Injury

• DOI: 10.1161/01.str.30.1.134
• Publication Date: 2011-06-17T20:08:03Z
• Authors: Sulpicio G. Soriano; Angela Coxon; Yanming F. Wang; Matthew P. Frosch; Stuart A. Lipton; Paul R. Hickey; Tanya N. Mayadas
• Keywords: Extravasation; Evans Blue; Stroke; Infiltration (HVAC)

Macrophage-1 antigen (Mac-1) (CD11b/CD18), a leukocyte beta2 integrin, facilitates neutrophil adhesion, transendothelial migration, phagocytosis, and respiratory burst, all of which may mediate reperfusion-induced injury to ischemic brain tissue in conditions such as stroke. To determine the role Mac-1 during ischemia reperfusion brain, we analyzed effect transient focal cerebral mice genetically engineered with specific deficiency Mac-1.Transient ischemia/reperfusion was induced by occluding left middle artery for 3 hours followed 21-hour period Mac-1-deficient (n=12) wild-type (n=11) mice. Regional blood flow determined laser-Doppler flowmeter. Brain sections were stained 2% 2,3,5-triphenyltetrazolium chloride infarct volume. Neutrophil accumulation staining dichloroacetate esterase identify neutrophils.Compared cohort, had 26% reduction infarction volume (P<0.05). This associated 50%, but statistically insignificant, number extravasated neutrophils infarcted areas brains mutant There no differences regional between 2 groups.Mac-1 reduces infiltration cell death after ischemia. finding be related extravasation