Quantitative Analysis of Gene Expressions Related to Inflammation in Canine Spastic Artery After Subarachnoid Hemorrhage

• DOI: 10.1161/01.str.32.1.212
• Publication Date: 2011-06-17T20:08:03Z
• Authors: Yasuo Aihara; Hidetoshi Kasuya; Hideaki Onda; Tomokatsu Hori; Jun Takeda
• Keywords: Inflammation; Reverse Transcriptase Polymerase Chain Reaction; Gene Expression Profiling; Subarachnoid Hemorrhage; Extracellular Matrix; Disease Models, Animal; 03 medical and health sciences; Dogs; 0302 clinical medicine; Transforming Growth Factor beta; Basilar Artery; Animals; Cytokines; Vasospasm, Intracranial; Fibroblast Growth Factor 2; Collagen; Chemokines; Cell Adhesion Molecules

Background and Purpose —The possible role of inflammatory reaction the cerebral artery in pathogenesis vasospasm has been noted recent studies. We quantitatively measured levels expression genes related to inflammation spastic a canine double-hemorrhage model. Methods —Twenty dogs were assigned 4 groups: group D0, control; D2, killed 2 days after cisternal injection blood; D7, given double injections blood 7 first injection; D14. Angiography was performed twice: on day before animals killed. Total RNA extracted from basilar artery. The expressions interleukin (IL)-1α, IL-6, IL-8, IL-10, tumor necrosis factor-α, E-secretin, fibronectin, intercellular adhesion molecule (ICAM)-1, vascular cell molecule-1, transforming growth factor-β, basic fibroblast factor, collagen types I, III, IV examined with TaqMan real-time quantitative reverse transcription–polymerase chain reaction. Results —Prolonged arterial narrowing peaking observed. There significant difference vessel caliber between D14 groups ( P <0.0001). differences mRNA for IL-1α, ICAM-1, type I =0.0079, 0.0196, 0.0040, 0.0017, <0.0001, respectively). average level highest D7 ICAM-1 (17-, 16-, 131-, 1.7-fold compared those respectively) (10.9-fold). Conclusions —Increased suggests that is associated sustained contraction.