Abstract 476: Negligible Impacts Of Anesthesia On The Morphodynamics Of Individual Red Blood Cells What Is Good News For Vessels: A Study Exploiting Holotomography

• DOI: 10.1161/atvb.43.suppl_1.476
• Publication Date: 2023-10-02T14:09:33Z
• Authors: Monika Chaszczews...; Agnieszka Antonczyk; Katarzyna Gorna; Zdzislaw Kielbowicz; Katarzyna Bogunia...; Przemyslaw Przadka
• Keywords: Beach morphodynamics; Premedication

Rationale: The shape and membrane dynamics of individual red blood cells (RBCs) are known to play a significant role in their functionality. morphodynamics RBCs optimizes flow properties vessels capillaries, which is important for the balance optimal distribution nutrients tissues. It also crucial pathological contexts because changes can influence vascular microvascular hemodynamics. dysregulate function through modulation oxygen delivery scavenging generation nitric oxide. Therefore, ideal conditions essential healthy function. Anesthetics, sedatives, analgesics, fluids used during anesthesia impact RBCs. same applies pneumoperitoneum performed laparoscopic procedures. Objectives: Taking above we aimed prospectively evaluate on RBC volume (CV, fL), surface area (SA, μm2), sphericity (SI), dry mass (CH, pg), Hb concentration (CHC, g/dL) fluctuation (nm). Methods: We exanimated (n=760) from 5 female dogs undergoing laparoscopic-assisted ovariohysterectomy (OVH). was collected 15 min after premedication with medetomidine 1h full (isoflurane, fentanyl). Measurements parameters using non-invasive, label-free method imaging holotomography. Statistical significance determined Mann-Whitney test. Results: Morphodynamics parameter values were stable. observe statistically no vs. one hour anesthesia: CH 24.20 24,19, p=0.57; CV 59.17 59.04, p=0.89; CHC 42.33 41.53, p=0.60; SA 117.38 119.56, p=0.13; SI 0.63 0.62, p=0.31; 68.18 65.18, p=0.95. Conclusions: Procedures related OVH not reflected well-being. This provides new insight into safety anesthetic protocol this study cell function, translates lack risk damage.