Endothelial Outgrowth Cells Are Not Derived From CD133 + Cells or CD45 + Hematopoietic Precursors

• DOI: 10.1161/atvbaha.107.144972
• Publication Date: 2007-05-11T01:40:33Z
• Authors: Frank Timmermans; Filip Van Hauwerm...; Magda De Smedt; Robrecht Raedt; Frank Plasschaert; Marc L. De Buyzere; Thierry C. Gillebert; Jean Plum; Bart Vandekerckhove
• Keywords: Male; 0301 basic medicine; Adolescent; Infant, Newborn; Endothelial Cells; Bone Marrow Cells; Cell Differentiation; Cell Separation; Fetal Blood; Flow Cytometry; Hematopoietic Stem Cells; 03 medical and health sciences; Antigens, CD; Humans; Leukocyte Common Antigens; Female; AC133 Antigen; Child; Peptides; Biomarkers; Cells, Cultured; Glycoproteins

Objective— Two types of endothelial progenitor cells (EPCs), early EPCs and late (also called outgrowth [EOCs]), were described in vitro previously. In this report, we dissect the phenotype precursor(s) that generate these cell with focus on markers CD34, CD133, vascular growth factor receptor-2 (VEGFR2) have been used to identify putative circulating precursors. We also included CD45 analysis assess relation between CD34 + hematopoietic progenitors (HPC), precursors, both generated EPC types. Addressing issue might lead a better understanding lineage give rise may contribute consensus their flowcytometric enumeration. Methods Results— Using sorting human cord blood (UCB) bone marrow (BM) cells, demonstrate EOC generating precursors are confined small − fraction, but not HPC nor any other subpopulation. monocytic displayed characteristics typical for EPCs. Phenotypic showed express VEGFR2 whereas CD133 as expected, VEGFR2. Conclusion— EOCs derived from or