Human ApoA-I Transfer Attenuates Transplant Arteriosclerosis via Enhanced Incorporation of Bone marrow–derived Endothelial Progenitor Cells

• DOI: 10.1161/atvbaha.107.158741
• Publication Date: 2007-12-07T01:53:41Z
• Authors: Yingmei Feng; Frank Jacobs; Eline Van Craeyveld; Christine Brunaud; Jan Snoeys; Marc Tjwa; Sophie Van Linthout; Bart De Geest
• Keywords: Arteriosclerosis; Human bone; Progenitor; Bone marrow transplant

Transplant arteriosclerosis is the leading cause of graft failure and death in patients with heart transplantation. Endothelial progenitor cells (EPCs) contribute to endothelial regeneration allografts. We investigated whether increased HDL cholesterol induced by adenoviral human apoA-I (AdA-I) transfer increases number function EPCs, promotes incorporation EPCs Balb/c allografts transplanted paratopically C57BL/6 ApoE-/- mice, attenuates transplant arteriosclerosis.EPC mice was after AdA-I as evidenced 1.5-fold (P<0.01) higher Flk-1 Sca-1-positive 1.4-fold DiI-acLDL isolectin-positive spleen cells. In addition, enhanced EPC vitro. Incorporation bone marrow-derived 5.8-fold at day 21 transplantation AdA-I-treated apoE-/- compared control mice. Enhanced a 2.6-fold increase CD31-positive resulted (P=0.059) reduction neointima 3.9-fold luminal area.Human circulating enhances their into allografts, regeneration, formation murine model arteriosclerosis.