Resveratrol Improves Endothelial Function

• DOI: 10.1161/atvbaha.109.187146
• Publication Date: 2009-05-29T01:55:18Z
• Authors: Hanrui Zhang; Jing Zhang; Zoltan Ungvari; Cuihua Zhang
• Keywords: Apocynin; Endothelial Dysfunction; Sodium nitroprusside; NAD(P)H oxidase

Oxidative stress plays an important role in type 2 diabetes-related endothelial dysfunction. We hypothesized that resveratrol protects against oxidative stress-induced dysfunction aortas of diabetic mice by inhibiting tumor necrosis factor alpha (TNFalpha)-induced activation NAD(P)H oxidase and preserving phosphorylation nitric oxide synthase (eNOS).We examined endothelial-dependent vasorelaxation to acetylcholine (ACh) (Lepr(db)) normal controls (m Lepr(db)). Relaxation ACh was blunted Lepr(db) compared with m Lepr(db), whereas endothelial-independent sodium nitroprusside (SNP) comparable. Resveratrol improved ACh-induced without affecting dilator response SNP. Impaired relaxation partially reversed incubating the vessels inhibitor apocynin a membrane-permeable superoxide dismutase mimetic TEMPOL. Dihydroethidium (DHE) staining showed elevated (O(2)(.-)) production both significantly reduced O(2)(.-) signal. increased nitrite/nitrate levels eNOS (Ser1177) phosphorylation, attenuated H(2)O(2) nitrotyrosine (N-Tyr) content aortas. Furthermore, mRNA protein expression TNFalpha. Genetic deletion TNFalpha (db(TNF-)/db(TNF-)) associated activity vascular suggesting pivotal aortic diabetes inducing reducing NO bioavailability.Resveratrol restored function TNFalpha-induced potential for new treatment approaches promote health metabolic diseases.