Variations in Platelet Proteins Associated With ST-Elevation Myocardial Infarction

• DOI: 10.1161/atvbaha.111.235713
• Publication Date: 2011-09-16T11:08:28Z
• Authors: Andrés F. Parguiña; Lilian Grigorian-...; Rosa M. Agra; Diego López-Otero; Isaac Rosa; Jana Alonso; Elvis Teijeira-Fe...; José Ramón Gonzál...; Ángel García
• Keywords: Blood Platelets; Male; Proteomics; 0301 basic medicine; Integrins; 0303 health sciences; Myocardial Infarction; Nuclear Proteins; Blood Proteins; Platelet Membrane Glycoproteins; Middle Aged; Protein Serine-Threonine Kinases; Platelet Activation; 3. Good health; Electrocardiography; 03 medical and health sciences; Case-Control Studies; Humans; Female; Prospective Studies; Acute Coronary Syndrome; Adaptor Proteins, Signal Transducing; Aged; Follow-Up Studies

Objective— Our aim in this study was to provide novel information on the molecular mechanisms playing a major role in the unwanted platelet activation associated with ST-elevation myocardial infarction (STEMI).

Methods and Results— We compared the platelet proteome of 11 STEMI patients to a matched control group of 15 stable chronic ischemic cardiopathy patients. In addition, we did a prospective study to follow the STEMI patients over time. Proteins were separated by high-resolution 2D gel electrophoresis, identified by mass spectrometry, and validated by Western blotting. Platelets from STEMI patients on admission displayed 56 protein spot differences (corresponding to 42 unique genes) compared with the control group. The number of differences decreased with time during the patients' follow-up. Interestingly, the adapter protein CrkL and the active form of Src (phosphorylated in Tyr418) were found to be upregulated in platelets from STEMI patients. Major signaling pathways related to the proteins identified include integrin, integrin-linked kinase, and glycoprotein VI (GPVI) signaling. Interestingly, a study on an independent cohort of patients showed a higher degree of activation of GPVI signaling in STEMI patients.

Conclusion— CrkL, the active form of Src, and GPVI signaling are upregulated in platelets from STEMI patients.