Deregulation of Retinaldehyde Dehydrogenase 2 Leads to Defective Angiogenic Function of Endothelial Colony–Forming Cells in Pediatric Moyamoya Disease

• DOI: 10.1161/atvbaha.115.305363
• Publication Date: 2015-05-29T02:56:52Z
• Authors: Ji Yeoun Lee; Youn Joo Moon; Hae-Ock Lee; Ae-Kyung Park; Seung-Ah Choi; Kyu-Chang Wang; Jung Woo Han; Je-Gun Joung; Hyun Seung Kang; Jeong Eun Kim; Ji Hoon Phi; Woong-Yang Park; Seung-Ki Kim
• Keywords: Pathogenesis; Matrigel

Objective–– Moyamoya disease (MMD) is a common cause of childhood stroke, in which the abnormal function endothelial colony–forming cell (ECFC) plays key role pathogenesis disease. This study was designed to identify genes involved MMD using gene expression profiling and understand defective ECFCs. Approach Results–– We compared profiles ECFCs isolated from patients with normal controls. Among differentially expressed genes, we selected most downregulated expression, retinaldehyde dehydrogenase 2 ( RALDH2 ). The activity assessed by vitro tube formation assay vivo Matrigel plug presence all-trans retinoic acid. transcriptional control tested ChIP assays on acetyl-histone H3. In results, inefficiently formed capillary tubes capillaries vivo, defect restored acid treatment. Knockdown mRNA also induced decreased vitro. level attributed H3 binding promoter region. Conclusions–– From these conclude that epigenetically suppressed MMD, may play their functional impairment.