Hematopoietic ChemR23 (Chemerin Receptor 23) Fuels Atherosclerosis by Sustaining an M1 Macrophage-Phenotype and Guidance of Plasmacytoid Dendritic Cells to Murine Lesions—Brief Report

• DOI: 10.1161/atvbaha.119.312386
• Publication Date: 2019-02-21T10:02:24Z
• Authors: Emiel P.C. van de...; Manuela Mandl; Madeleine Müller; Carlos Neideck; Yvonne Jansen; Michael Hristov; Selin Gencer; Linsey J.F. Peters; Svenja Meiler; Micha Feld; Anna-Lena Geiselh...; Renske J. de Jong; Caspar Ohnmacht; Heidi Noels; Oliver Soehnlein; Maik Drechsler; Christian Weber; Yvonne Döring
• Keywords: EXPRESSION; Male; 0301 basic medicine; mice; Mice, Knockout, ApoE; Atherosclerosis ; Chemokines ; Chemokine Receptor ; Dendritic Cells ; Macrophages ; Mice; chemokines; Gene Knockout Techniques; Mice; 03 medical and health sciences; INFLAMMATION; Genes, Reporter; Cell Adhesion; Animals; dendritic cells; Gene Knock-In Techniques; Inflammation; Macrophages; chemokine receptor; Dendritic Cells; Macrophage Activation; Atherosclerosis; macrophages; 3. Good health; Mice, Inbred C57BL; Cholesterol; FAT; Diet, Western; Disease Progression; Intercellular Signaling Peptides and Proteins; Female; atherosclerosis; Chemokines

Objective— Expression of the chemokine-like receptor ChemR23 (chemerin receptor 23) has been specifically attributed to plasmacytoid dendritic cells (pDCs) and macrophages and ChemR23 has been suggested to mediate an inflammatory immune response in these cells. Because chemokine receptors are important in perpetuating chronic inflammation, we aimed to establish the role of ChemR23-deficiency on macrophages and pDCs in atherosclerosis.

Approach and Results—

ChemR23-knockout/knockin mice expressing eGFP (enhanced green fluorescent protein) were generated and after crossing with apolipoprotein E-deficient ( Apoe −/− ChemR23

e/e

) animals were fed a western-type diet for 4 and 12 weeks. Apoe −/− ChemR23

e/e

mice displayed reduced lesion formation and reduced leukocyte adhesion to the vessel wall after 4 weeks, as well as diminished plaque growth, a decreased number of lesional macrophages with an increased proportion of M2 cells and a less inflammatory lesion composition after 12 weeks of western-type diet feeding. Hematopoietic ChemR23-deficiency similarly reduced atherosclerosis. Additional experiments revealed that ChemR23-deficiency induces an alternatively activated macrophage phenotype, an increased cholesterol efflux and a systemic reduction in pDC frequencies. Consequently, expression of the pDC marker SiglecH in atherosclerotic plaques of Apoe −/− ChemR23

e/e

mice was declined. ChemR23-knockout pDCs also exhibited a reduced migratory capacity and decreased CCR (CC-type chemokine receptor)7 expression. Finally, adoptive transfer of sorted wild-type and knockout pDCs into Apoe −/− recipient mice revealed reduced accumulation of ChemR23-deficient pDCs in atherosclerotic lesions.

Conclusions— Hematopoietic ChemR23-deficiency increases the proportion of alternatively activated M2 macrophages in atherosclerotic lesions and attenuates pDC homing to lymphatic organs and recruitment to atherosclerotic lesions, which synergistically restricts atherosclerotic plaque formation and progression.