A New Autosomal Myh11-CreER T2 Smooth Muscle Cell Lineage Tracing and Gene Knockout Mouse Model—Brief Report

• DOI: 10.1161/atvbaha.122.318160
• Publication Date: 2022-12-15T10:03:04Z
• Authors: Rebecca A. Deaton; Gamze Bulut; Vlad Serbulea; Anita Salamon; Laura S. Shankman; Anh Tram Nguyen; Gary K. Owens
• Keywords: Gene knockout

Background: The Myh11 promoter is extensively used as a smooth muscle cell (SMC) Cre-driver and regarded the most restrictive specific available to study SMCs. Unfortunately, in existing Myh11-CreER T2 mouse, transgene was inserted on Y chromosome precluding of female mice. Given importance including sex biological variable that numerous SMC-based diseases have sex-dependent bias, field has been tremendously limited by lack model both sexes. Here, we describe new autosomal mouse (referred -RAD ), which allows for SMC-specific lineage tracing gene knockout studies vivo using male Methods: A transgenic C57BL/6 line generated bacterial artificial clone RP23-151J22 modified contain Cre-ER after start codon. mice were crossed with 2 different fluorescent reporter tested labeling flow cytometric immunofluorescence analyses. Results: insertion determined be 2. showed Cre-dependent, tamoxifen-inducible SMCs equivalent widely Labeling Cre + vascular visceral pericytes various tissues assessed immunofluorescence. Conclusions: We validated function an can assess respect normal pathophysiological functions