Abstract 18166: Increasing Endothelialization of BMS and DES With Intra-arterial AdVEGF-A Gene Therapy in Porcine Coronary Arteries

• DOI: 10.1161/circ.132.suppl_3.18166
• Publication Date: 2022-03-19T21:52:40Z
• Authors: Jarkko P Hytonen; Paavo Halonen; Santeri Tarvainen; Jouni Taavitsainen; Antti Kuivanen; Arto Koistinen; Johanna Laakkonen; Juha Hartikainen; Seppo Yla-Herttuala
• Keywords: Angioscopy; Ex vivo; Coronary arteries

We aimed to increase stent endothelialization with gene therapy known promote endothelial proliferation. In addition, we evaluated OCT and angioscopy imaging as tools for detecting endothelium comparison robust ex-vivo analyses including scanning electron microscopy (SEM), multiphoton immunohistology. 24 stents, BMS DES, were implanted into naïve porcine coronary arteries received either AdVEGF-A or control AdLacZ a porous drug delivery catheter. Stents imaged in-vivo angiography, immediately after stenting one week two weeks therapy. At d14 the stents collected histology, multi-photon assessment of endothelialization. Strut coverage was analyzed from SEM images graded 0 (no coverage) 3 (fully covered). Angioscopy pullbacks thrombus in stented artery similarly thrombus) (occlusive thrumbus). Initial intervention showed increased strut VEGF-A compared LacZ (2.8±0.4 vs. 1.8±0.4, p=0.0031, respectively, a) b) figure). Gene did not change DES 1.6±0.5 p=NS, respectively. Angioscopic formation low all groups trend towards less thrombi on (0.2±0.4 0.3±0.5 AdVEGF-A) (0.8±0.8 0.8±0.7 AdVEGF, c) figure red at 4 o'clock). treatment time but improve healing stenting. Likely strong cytotoxic drugs hamper even local supraphysiological growth factor concentrations. may be useful arterial stent.