Abstract 10630: Perfusable Apparatus for Thick-Tissue Creation and Growth (patch) of Cardiac Tissue

• DOI: 10.1161/circ.144.suppl_1.10630
• Publication Date: 2022-03-19T23:58:21Z
• Authors: Tyler Dunn; Katherine Nurminsky; Isaree Pitaktong; Yusheng Jason He; Sarah Koljaka; Olivia Dunne; Stephanie Ran; Wesley Shih; Anya Wang; Hiroshi Matsushita; Daniel Rodgers; Narutoshi Hibino
• Keywords: 03 medical and health sciences; 0302 clinical medicine

Background: Cardiac tissue engineering has beendeveloped as apotential alternativetreatment for heart failure. However, current 3D tissues are limited insize and thickness due tothe lack of aneffective vascularizationmethod. We have developed anovel bioreactor systemtocreate viable vascularized cardiac from multicellular spheroids using adigital lightprocessing (DLP)3D bioprinting system. Methods: Spheroids were created induced pluripotent stem cells (iPSC)and cardiacfibroblasts (FB)using special dimple plates mass production. One centimeter cubic tissueswere aDLP bioprinted mold with vascular channels. Thetissue was maintained inaperfusionchamber under regulated flow pressure followingdifferentiationtocardiac endothelialization. Results: Mass productionof large (35,000/ tissue, diameter 395.99um +/- 101.15um)was achieved 170millioniPSCs 50millionFBs the creationof 1cm3cardiactissue inabioprinted The (n=5)were perfused for20days stable 17.5+/- 3.05PSI 5000uL/min+/- 1116.42uL/min.Ondays 10and 20, Alamar blue assays showed viability all five (Alamar blueintensity: Day 101.57+/- 0.15. 202.21+/- 0.19). Conclusion: Thick a3Dbioprinted perfusionsystem maturationand growth invitrofor 20days. This technology will opennew doors invitrocardiac creation.