MicroRNA-133 Controls Vascular Smooth Muscle Cell Phenotypic Switch In Vitro and Vascular Remodeling In Vivo

• DOI: 10.1161/circresaha.111.240150
• Publication Date: 2011-08-19T04:40:23Z
• Authors: Daniele Torella; Claudio Iaconetti; Daniele Catalucci; Georgina M. Ellison; Angelo Leone; Cheryl D. Waring; Angela Bochicchio; Carla Vicinanza; Iolanda Aquila; Antonio Curcio; Gianluigi Condorelli; Ciro Indolfi
• Keywords: Male; Myocytes; 0303 health sciences; Myocytes, Smooth Muscle; Wistar; Carotid Artery Injuries ; Cell Proliferation ; Male ; MicroRNAs; Muscle, Smooth, Vascular ; Myocytes, Smooth Muscle ; Rats; 610; Muscle, Smooth, Vascular; Rats; MicroRNAs; 03 medical and health sciences; Phenotype; Smooth Muscle; Vascular; 616; Muscle; Animals; Smooth; Rats, Wistar; Carotid Artery Injuries; Cell Proliferation

MicroRNA (miR)-1 and -133 play a crucial role in skeletal cardiac muscle biology pathophysiology. However, their expression regulation vascular cell physiology disease is currently unknown.The aim of the present study was to evaluate role, if any, miR-1 miR-133 smooth (VSMC) phenotypic switch vitro vivo.We demonstrate here that robustly expressed cells (VSMCs) vivo, whereas levels are negligible. has potent inhibitory on VSMC does not have any relevant effect per se. regulated by extracellular signal-regulated kinase 1/2 activation inversely correlated with growth. Indeed, decreases when VSMCs primed proliferate following injury it increases coaxed back quiescence vivo. loss- gain-of-function experiments show plays mechanistic Accordingly, adeno-miR-133 reduces but anti-miR-133 exacerbates proliferation migration specifically suppresses transcription factor Sp-1 vivo through repression regulates gene expression.Our data key regulator suggesting its potential therapeutic application for diseases.