CD44 Binds to Macrophage Mannose Receptor on Lymphatic Endothelium and Supports Lymphocyte Migration via Afferent Lymphatics

• DOI: 10.1161/circresaha.111.300476
• Publication Date: 2013-04-20T03:36:11Z
• Authors: Marko Salmi; Marika Karikoski; Kati Elima; Pia Rantakari; Sirpa Jalkanen
• Keywords: Lymphocyte homing receptor; High endothelial venules; Lymphatic Endothelium; Mannose receptor; Lymphatic vessel

Macrophage mannose receptor (MRC) is one of the few molecules known to be involved in lymphocyte trafficking via lymphatic vessels. In endothelial cells efferent lymphatics, it binds L-selectin on lymphocytes. afferent MRC mediates both normal and malignant L-selectin-negative draining lymph nodes.This work was designed search for additional ligands elucidate how lymphocytes migrate into nodes.Using immunoprecipitation binding studies with natural recombinant proteins, we show that CD44 can interact each other. Fine mapping revealed cysteine-rich domain chondroitin sulfate side chains CD44. vivo homing experiments MRC- CD44-deficient mice verified function as a receptor-ligand pair supporting migration lymphatics nodes.These data identify new counter-receptor reveal molecule poorly understood process transit vasculature.