Functional Role of Matrix Metalloproteinase-8 in Stem/Progenitor Cell Migration and Their Recruitment Into Atherosclerotic Lesions

• DOI: 10.1161/circresaha.112.274019
• Publication Date: 2012-10-16T06:41:05Z
• Authors: Qingzhong Xiao; Feng Zhang; Luyang Lin; Changcun Fang; Guanmei Wen; Tsung-Neng Tsai; Xiangyuan Pu; David Sims; Zhongyi Zhang; Xiaoke Yin; Binia Thomaszewski; Boris Schmidt; Manuel Mayr; Ken Suzuki; Qingbo Xu; Shu Ye
• Keywords: Matrigel

Accumulating evidence indicates that stem/progenitor cells (SPCs) represent an important source of in atheromas and contribute to lesion formation progression.We investigated whether matrix metalloproteinase-8 (MMP8) played a role SPC migration their recruitment into atheromas.We found SPCs expressed MMP8 knockout significantly reduced numbers atherosclerotic lesions apolipoprotein E (ApoE)-deficient mice fed Western diet. Further vivo experiments showed ApoE(-/-)/MMP8(-/-) injected with stem isolated from bone marrows had fewer smaller than ApoE(-/-)/MMP8(+/+) mice. Ex deficiency inhibited the ability migrate arterial lumen adventitia lesions. In vitro assays indicated facilitated across endothelial through Matrigel or collagen I. We also cleaved a-disintegrin-and-metalloproteinase-domain-10 mature on SPCs. Knockdown incubation inhibitor GI254023X decreased E-cadherin shedding The decrease migratory knockdown was by such culture supernatant without knockdown, this compensatory effect abolished antibody against soluble E-cadherin.MMP8 plays