Postnatal Cardiac Gene Editing Using CRISPR/Cas9 With AAV9-Mediated Delivery of Short Guide RNAs Results in Mosaic Gene Disruption

• DOI: 10.1161/circresaha.116.310370
• Publication Date: 2017-08-30T00:50:16Z
• Authors: Anne Katrine Joha...; Bas Molenaar; Danielle Versteeg; Ana Rita Leitoguinho; Charlotte Demkes; Bastiaan Spanjaard; Hesther de Ruiter; Farhad Akbari Moq...; Lieneke Kooijman; Lorena Zentilin; Mauro Giacca; Eva van Rooij
• Keywords: Guide RNA

CRISPR/Cas9 (clustered regularly interspaced palindromic repeats/CRISPR-associated protein 9)-based DNA editing has rapidly evolved as an attractive tool to modify the genome. Although been extensively used manipulate germline in zygotes, its application postnatal gene remains incompletely characterized.