Induced Pluripotent Stem Cell (iPSC)–Derived Extracellular Vesicles Are Safer and More Effective for Cardiac Repair Than iPSCs

• DOI: 10.1161/circresaha.117.311769
• Publication Date: 2017-11-08T10:15:15Z
• Authors: Marta Adamiak; Guangming Cheng; Sylwia Bobis-Wozo...; Lin Zhao; Sylwia Kedracka-Krok; Anweshan Samanta; Elzbieta Karnas; Yu-Ting Xuan; Bozena Skupien-Ra...; Xing Chen; Urszula Jankowska; Magdy Girgis; Malgorzata Sekula; Arash Davani; Slawomir Lasota; Robert J. Vincent; Michal Sarna; Kathy L. Newell; Ou-Li Wang; Nathaniel Dudley; Zbigniew Madeja; Buddhadeb Dawn; Ewa K. Zuba-Surma
• Keywords: Male; 0301 basic medicine; induced pluripotent stem cells; Cell Survival; Induced Pluripotent Stem Cells; apoptosis; Myocardial Infarction; Mice, Transgenic; Myocardial Reperfusion Injury; 3. Good health; Mice, Inbred C57BL; angiogenesis; Extracellular Vesicles; Mice; 03 medical and health sciences; myocardial infarction; Treatment Outcome; stem cells; Cell Movement; Animals; Myocytes, Cardiac; extracellular vesicles; Cells, Cultured; remodeling

Rationale:

Extracellular vesicles (EVs) are tiny membrane-enclosed droplets released by cells through membrane budding or exocytosis. The myocardial reparative abilities of EVs derived from induced pluripotent stem cells (iPSCs) have not been directly compared with the source iPSCs.

Objective:

To examine whether iPSC-derived EVs can influence the biological functions of cardiac cells in vitro and to compare the safety and efficacy of iPSC-derived EVs (iPSC-EVs) and iPSCs for cardiac repair in vivo.

Methods and Results:

Murine iPSCs were generated, and EVs isolated from culture supernatants by sequential centrifugation. Atomic force microscopy, high-resolution flow cytometry, real-time quantitative RT-PCR, and mass spectrometry were used to characterize EV morphology and contents. iPSC-EVs were enriched in miRNAs and proteins with proangiogenic and cytoprotective properties. iPSC-EVs enhanced angiogenic, migratory, and antiapoptotic properties of murine cardiac endothelial cells in vitro. To compare the cardiac reparative capacities in vivo, vehicle, iPSCs, and iPSC-EVs were injected intramyocardially at 48 hours after a reperfused myocardial infarction in mice. Compared with vehicle-injected mice, both iPSC- and iPSC-EV–treated mice exhibited improved left ventricular function at 35 d after myocardial infarction, albeit iPSC-EVs rendered greater improvement. iPSC-EV injection also resulted in reduction in left ventricular mass and superior perfusion in the infarct zone. Both iPSCs and iPSC-EVs preserved viable myocardium in the infarct zone, whereas reduction in apoptosis was significant with iPSC-EVs. iPSC injection resulted in teratoma formation, whereas iPSC-EV injection was safe.

Conclusions:

iPSC-derived EVs impart cytoprotective properties to cardiac cells in vitro and induce superior cardiac repair in vivo with regard to left ventricular function, vascularization, and amelioration of apoptosis and hypertrophy. Because of their acellular nature, iPSC-EVs represent a safer alternative for potential therapeutic applications in patients with ischemic myocardial damage.