Notch Activation of Jagged1 Contributes to the Assembly of the Arterial Wall

• DOI: 10.1161/circulationaha.111.047159
• Publication Date: 2011-12-07T05:38:49Z
• Authors: Lauren J. Manderf...; Frances A. High; Kurt A. Engleka; Feiyan Liu; Li Li; Stacey Rentschler; Jonathan A. Epstein
• Keywords: JAG1

Background— Notch signaling in vascular smooth muscle precursors is required for differentiation. Jagged1 expression on endothelium activates including those of neural crest origin to initiate the formation a layer maturing blood vessel. Methods and Results— Here, we show that direct target muscle, resulting positive feedback loop lateral induction propagates wave differentiation during aortic arch artery development. In vivo, inhibition cardiac impairs messenger RNA results deficient resultant defects. Ex ligand explants activation RNA, response blocked by inhibition. We examine 15 evolutionary conserved regions within genomic locus identify single element second intron. This contains functional Rbp-J binding site demonstrated luciferase reporter chromatin immunoprecipitation assays sufficient recapitulate transgenic mice. Loss Conclusions— Taken together, these provide mechanism allows multilayered wall form around nascent arterial endothelial tube as target.