Intracoronary Delivery of Autologous Cardiac Stem Cells Improves Cardiac Function in a Porcine Model of Chronic Ischemic Cardiomyopathy

• DOI: 10.1161/circulationaha.112.001075
• Publication Date: 2013-06-12T01:00:44Z
• Authors: Roberto Bolli; Xian-Liang Tang; Santosh K. Sangan...; Ornella Rimoldi; Federico Mosna; Ahmed Abdel-Latif; Hani Jneid; Marcello Rota; Annarosa Leri; Jan Kajstura
• Keywords: Ischemic Cardiomyopathy; Troponin T; Troponin complex

Relevant preclinical models are necessary for further mechanistic and translational studies of c-kit+ cardiac stem cells (CSCs). The present study was undertaken to determine whether intracoronary CSCs beneficial in a porcine model chronic ischemic cardiomyopathy.Pigs underwent 90-minute coronary occlusion followed by reperfusion. Three months later, autologous (n=11) or vehicle (n=10) were infused into the infarct-related artery. At this time, all indices left ventricular (LV) function similar control CSC-treated pigs, indicating that damage inflicted infarct 2 groups similar; 1 month however, pigs exhibited significantly greater LV ejection fraction (echocardiography) (51.7±2.0% versus 42.9±2.3%, P<0.01), systolic thickening infarcted wall, maximum dP/dt, as well lower end-diastolic pressure. Confocal microscopy showed clusters small α-sarcomeric actin-positive expressing Ki67 scar treated consistent with regeneration. origin these cycling myocytes from injected confirmed 4 received enhanced green fluorescent protein -labeled CSCs, which positive markers troponin I, T, myosin heavy chain, connexin-43. Some engrafted also formed vascular structures expressed α-smooth muscle actin.Intracoronary infusion improves regional global promotes regeneration old myocardial infarction (scar). results mimic those recently reported humans (Stem Cell Infusion Patients Ischemic CardiOmyopathy [SCIPIO] trial) establish cardiomyopathy useful clinically relevant studying CSCs.