Abstract 085: The Role of Afferent and Efferent Renal Nerves in T Lymphocyte Recruitment into the Kidneys and Development of Two Models of Salt-Dependent Hypertension

• DOI: 10.1161/hyp.64.suppl_1.085
• Publication Date: 2021-07-03T09:13:05Z
• Authors: Jason D Foss; Jessica Fiege; Dusty Van Helden; Yoji Shimizu; John P Collister; Tim Mayerhofer; Laurel Wood; John W Osborn
• Keywords: 03 medical and health sciences; 0302 clinical medicine

Renal denervation (RDNX) attenuates hypertension (HT) in some animal models and human patients, yet the antihypertensive mechanisms involved are not known. Because it has been suggested that efferent renal nerves may cause recruitment of T lymphocytes (T cells) into kidneys, cell-secreted cytokines can activate afferent neurons, we hypothesized RDNX HT angiotensin II (AngII)-salt deoxycorticosterone acetate (DOCA)-salt by decreasing nerve-dependent cell trafficking to kidneys cell-mediated activation nerves. Male Sprague-Dawley rats underwent SHAM surgery, or selective ablation (renal-CAP) were subjected either AngII-salt DOCA-salt HT. SHAM, renal-CAP (n = 8/group) instrumented with an IV catheter radio-telemeter measure mean arterial pressure (MAP). Rats fed a 4% NaCl diet and, after baseline period, AngII was infused (10 ng/kg/min) for 16 days. unilaterally nephrectomized 2 weeks, radio-telemeter, 5), 7) 6) given 0.9% saline drink. After silicone pellets containing 100mg DOCA implanted SC sacrificed 3 weeks later. Upon sacrifice, harvested flow cytometry determine number CD4+ CD8+ cells kidneys. In rats, CD4 CD8 counts change MAP from (ΔMAP) similar between groups (SHAM 42.3 ± 6.4 mmHg, 32.6 5.9 36.8 5.0 mmHg). significantly attenuated ΔMAP 24.2 3.1 12.7 2.4 13.8 1.7 lower than (p < 0.01) trended renal-CAP. 0.05) SHAM. These data show do contribute HT, but suggest this be dependent on Funding: R01 HL116476-01.