Abstract 264: Nitrite Therapy Ameliorates Myocardial Injury via H2S and Nuclear Factor-Erythroid 2-Related Factor 2 (Nrf2)-Dependent Signaling in Chronic Heart Failure

• DOI: 10.1161/res.117.suppl_1.264
• Publication Date: 2021-07-03T09:14:18Z
• Authors: Kazi N Islam; Erminia Donnarumma; Shashi Bhushan; David J Lefer
• Keywords: 0303 health sciences; 03 medical and health sciences; 3. Good health

Background: Nitric oxide (NO) and hydrogen sulfide (H 2 S) are reduced in congestive heart failure. Recent studies suggest cross-talk between NO H S signaling. We previously reported that sodium nitrite (NaNO ) significantly ameliorates myocardial ischemia-reperfusion injury Nrf2 regulates the expression of antioxidant protein genes is upregulated by S. examined effects NaNO therapy on endogenous bioavailability activation mice subjected to ischemia-induced Materials Methods: Mice underwent 60 min. left coronary artery occlusion 4 weeks (WKS) reperfusion. (165 μg/kg) or saline vehicle (VEH) was administered at reperfusion then drinking water (100 mg/L) for wks. Left ventricular ejection fraction (LVEF) determined baseline wks Myocardial tissue collected analyzed oxidative stress status respective gene/protein levels. Results: preserved LVEF (47 ± 4% vs. 32 4%, p < 0.01) LV diastolic systolic dimensions (LVEDD/LVESD; 4.0/3.1 mm 4.5/3.9 mm, 0.05) MDA carbonyl contents were treated as compared VEH. markedly increased CuZn-superoxide dismutase catalase Furthermore, mRNA levels producing enzymes bioavailabilty. Cardiac also observed with therapy. Conclusions: Our results demonstrate improves function via increasing bioavailability, Nrf2, defenses.1