Abstract WP498: Impaired Pericyte Constriction and Cerebral Blood Flow Autoregulationin Diabetes

• DOI: 10.1161/str.51.suppl_1.wp498
• Publication Date: 2021-06-21T20:32:19Z
• Authors: Yedan Liu; Shaoxun Wang; Ya Guo; Huawei Zhang; Richard Roman; Fan Fan
• Keywords: Pericyte

Diabetes is the primary pathological factor attributed to Alzheimer’s disease and vascular cognitive impairment. Previous studies demonstrated that hyperglycemia promoted oxidative stress in cerebral vasculature. Cerebrovascular pericytes contribute maintaining blood-brain barrier (BBB) integrity regulating blood flow (CBF). However, whether diminishes contractile capability of pericytes, impairs CBF autoregulation increases BBB permeability are unclear. In present study, we examined role cerebrovascular function cognition diabetes using cell culture vitro , isolated penetrating arterioles ex vivo . Reactive oxygen species were elevated high glucose (HG, 30 mM) treated vs. normal (NG, 5.5 pericytes. Further, mitochondrial superoxide production was increased HG-treated NG-treated group (13.24 ± 1.01 arbitrary unit (a.u.)/30min 6.98 0.36 a.u./30min). Mitochondrial respiration decreased (3718 185.9 pmol/min/mg, n=10 4742 284.5 n=10) as measured by a Seahorse XFe24 analyzer. displayed fragmented mitochondria association with fission protein (DRP1) fusion (OPA1) expression. lower than cells (20.23 7.15% 29.46 9.41%). The myogenic response impaired from diabetic rats comparison non-diabetic rats. Autoregulation laser Doppler flowmeter compared Diabetic exhibited greater leakage control an eight-arm water maze. took longer time escape indicating learning memory deficits. conclusion, induces pericyte dysfunction altering dynamics diminishing capability, which promotes leakage, decreases contributes diabetes-related dementia.