Abstract TP297: Focused Mouse Brain Irradiation Model to Define the Neurovascular Unit

• DOI: 10.1161/str.55.suppl_1.tp297
• Publication Date: 2024-02-01T10:11:28Z
• Authors: Roberto J Alcazar...; Abhinav Srinath; Akash Bindal; Ahmed Malik; Erik Pearson; Fidel Haro; Dominic Montas; Stephanie Hage; Peter Pytel; Issam Awad; Sean P Polster
• Keywords: Astrogliosis; Neurovascular bundle

Purpose: This project aimed to develop a stereotactic radiosurgery mouse model of radiation-induced brain injury study endothelial damage within the neurovascular unit (NVU). Classification focal and resulting inflammatory cascade has never been cataloged for assessing intrinsic changes versus extrinsic factors inflammation at NVU. The with invasive models, such as open transcranial or vessel occlusion, introduces multiple variables into system that our can help overcome secondary systemic insults Methods: Using an X-ray image-guided radiation therapy system, mice were focally irradiated 30, 45, 70 Gy 5 mm target. Mice followed up 2, 4, 6, 12 18 weeks after irradiation contrast-enhanced CT imaging volumetric quantification effects. Brains harvested characterization by histology immune-multiplexing reactionary Whole coronal slides scanned analyzed using machine learning classifiers quantify cell types NVU in radiated vs. control hemispheres. Results: Radiation-induced telangiectasias, hyalinization, white matter necrosis observed. An response predominance T cells was observed, well increase microglial reactive astrogliosis. These time dose-dependent radiation. Serial CTs allowed determining development damage, contrast enhancement proportional degree changes. Conclusions: allows precise targeting steep fall-off surrounding tissues. NVU’s insult presents novel method specifically diseases associated dysfunction.