Abstract TMP4: Serum Protein Biomarekrs and Cell Suptypes Linked to Reduced Cerebral Edema in Aneurysmal Subarachnoid Hemorrhage

• DOI: 10.1161/str.56.suppl_1.tmp4
• Publication Date: 2025-01-30T10:02:02Z
• Authors: Bosco Seong Kyu Yang; Aaron Gusdon; Xuefang Ren; H Choi
• Keywords: Cerebral edema; Brain Edema

Introduction: Early brain injury (EBI), a complex collection of pathophysiological processes occurring within 72 hours aneurysmal subarachnoid hemorrhage (aSAH), is the key link connecting initial event to delayed and long-term complications. Cerebral edema major constituent EBI, resulting from direct toxicity hemoglobin products critical increase in intracranial pressure. This study aims identify serum protein biomarkers specifically targeting cerebral EBI cell-specific interventional targets. Methods: based on prospective observational cohort at single tertiary referral center. Plasma samples collected three separate groups aSAH patients were analyzed. Each consisted 64, 71, 16 48 rupture. For first cohort, proximity extension assay by Olink was used analyze 184 proteins. second corhot, commercially available enzyme linked immunosorbent (ELISA) kits determine plasma concentrations candidate last peripheral blood mononuclear cells isolated their single-cell transcriptomic (scRNA) data extracted using 10X genomics platform. each patient, early score (SEBES) scans dichotomized with severe being defined as SEBES > 2. Results: Overall, median age 52.5 years [IQR:45-63] 76% female. 35% had edema. Subpopulations divided outcome did not differ significantly, except for Hunt-Hess grade (HH), 71% 32% without showing HH greater than three, respectively (p < 0.05). After adjusting multiplicity, ten proteins showed overlapping, lowest p-values, five chosen candidates mechanistic relevance. Among proteins, ALK1 LAYN statistically different expressions, milder higher expression. scRNA analysis that preferentially expressed cell cluster co-expresses CST3, CLEC10A, FCER1A but has lower levels markers known subtypes type 2 conventional dendritic cells. Conclusion: suggests existence specific subtype cells, potentially associated less following aSAH.